For a long time, the relationship between predators and prey has been one of the central topics of ecological research. Entomologists have used many different methods to study this relationship, hoping to correctly evaluate the control effect of predatory natural enemies on target pests. Among many methods, the direct evidence of predation is best obtained by serological methods. The theoretical basis of the serological method is that there are prey proteins in the digestive tract of natural enemies that can react with antibodies.
Serological tests have been used to analyze the contents of the digestive tract of predators for 40 years. The first methods used were precipitation test, two-way diffusion method, single-diffusion method, convection immunoelectrophoresis and cross immunoelectrophoresis. The precipitation test is neither sensitive nor specific. Because of the simple equipment, low cost, and easy interpretation of the test results, many people use it. With the continuous development of immunology technology, many new technologies have been continuously adopted and developed in the direction of high sensitivity and specificity. In the past ten years, people have cited indirect (passive) coagulation test, radioimmunoassay and enzyme-linked immunosorbent assay. The first two trials are not widely used because of their complexity, and the radioimmunoassay is expensive and unsafe.
The earliest introduction of ELISA into the study of predation was Fichter and Stephen. Since then, Ragsdale et al. Have used ELISA to study the predation effect of a series of predators (excluding spiders) on rice green stink bug (Nezaraviridura): Miller can use ELISA to distinguish Dendroctonus frontalis from many beetles And pointed out the potential application value of this method in the study of predator-prey relationship. At present, the work of Sunderland et al. Is the most effective to study the predation effect by ELISA. They have conducted extensive and in-depth studies on the predation effects of the wheat predators. Based on the field and laboratory research, the quantitative evaluation method of predation effect was summarized and improved. In addition, domestic methods such as removing sunflowers have used ELISA to qualitatively study the evaluation methods of the predatory natural enemies of blight insects, and analyzed the application value of ELISA based on the research results, and pointed out the problems to be further studied.
The above article was collated and published by Shanghai Yanjin Biotechnology Co., Ltd.
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