What is the difference between chlorophyll fluorescence spectrometer and chlorophyll meter?
Q: What is the difference between a chlorophyll fluorescence meter and a chlorophyll meter?
A: In daily business consultation, we are often asked by users about the above issues. In fact, the instruments represented by the above two names are quite different in terms of research purposes, measurement methods, methods of use, and objects of use.
For research purposes, when people choose fluorometers, they are mostly studying the mechanism of photosynthesis, such as the process and efficiency of electron transfer, the functions of photosynthetic reaction center II (PSII) and reaction center I (PSI), and environmental changes ( Arid, salt and alkali, metal pollution) for the purpose of electron transfer and so on. The chlorophyll meter is mainly used to judge the nutritional status (especially the nitrogen level) during plant growth, and provides a reference for crop fertilization. In summary, chlorophyll fluorescence meters are used for theoretical studies, while chlorophyll meters are used for applied research.
In the measurement method, the fluorometer uses the fluorescence phenomenon unique to chlorophyll, and uses a sensor with extremely high sensitivity and reaction speed, supplemented by a photoelectric pulse design to capture fluorescence data at various periods in the electron transfer process. Fluorescence measurement is a process measurement. The chlorophyll meter is a special absorption characteristic of chlorophyll for red light, and the amount of red light absorption by the photoelectric sensor is measured. The measurement of the chlorophyll meter is an instantaneous measurement at a certain point at a certain time.
In terms of usage, the use of the fluorometer requires long-term dark processing of the blade, while the chlorophyll meter requires time to make correction curves for different samples.
It can be seen from the above explanation that fluorometers are mostly used in research work of research institutes and universities. The chlorophyll meter is mostly used in agricultural extension stations and in the production technical guidance of agricultural technology stations.
Last but not least, the data obtained from the chlorophyll meter currently on the market is the relative content of chlorophyll rather than the absolute content. To detect the absolute content of chlorophyll in a sample, chemical colorimetry must be used.
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