Quantitative Detection Kit (ELISA) for Rat Heart-type Fatty Acid Binding Protein (H-FABP)
user's Guide
ã€Rat Heart Fatty Acid Binding Protein (H-FABP) Elisa Kit Kit Name】
Quantitative Detection Kit (ELISA) for Rat Heart-type Fatty Acid Binding Protein (H-FABP)
[Use of rat heart-shaped fatty acid binding protein (H-FABP) Elisa kit kit]
Quantitatively detect the content of central fatty acid binding protein (H-FABP) in rat serum, plasma and related liquid samples.
ã€Testing Principle of Rat Heart-shaped Fatty Acid Binding Protein (H-FABP) Elisa Kit】
This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the standard and the test sample to the pre-coated rat heart-type fatty acid binding protein (H-FABP) monoclonal antibody transparent enzyme label coated plate. After incubation for a sufficient time, wash to remove unbound components before adding Enzyme-labeled working solution, after incubation for a sufficient time, wash to remove unbound components. Substrates A and B were added in sequence, and the substrate (TMB) was converted into a blue product catalyzed by horseradish peroxidase (HRP), which turned yellow under the action of an acid. The concentration of fatty acid binding protein (H-FABP) was positively correlated. The OD value was measured at a wavelength of 450 nm. Based on the OD value of the standard and the sample, the content of rat heart-shaped fatty acid binding protein (H-FABP) in the sample was calculated.
[Composition of Elisa kit for rat heart-shaped fatty acid binding protein (H-FABP)]
1
Enzyme coated plate
12 holes × 8
7
Developer A liquid
6mL
2
Standard
0.3mL × 6 tubes
8
Developer B liquid
6mL
3
20 times concentrated washing liquid
25mL
9
Stop solution
6mL
4
Sample diluent
6mL
10
Instructions
1 serving
5
Special diluent
6mL
11
Sealing film
2 sheets
6
Enzyme reagent
6mL
12
sealed bag
1
Remarks: The concentration of standard products (No. 1 → No. 6) is: 1200, 600, 300, 150, 75, 37.5 pg / mL.
[Rat Heart Fatty Acid Binding Protein (H-FABP) Elisa kit required but not provided reagents and equipment]
1. 37 ℃ thermostat
2. Standard specification microplate reader
3. Precision pipettes and disposable tips
4. Distilled water
5. Disposable test tubes
6. Absorbent paper
[Procedures of rat heart-shaped fatty acid binding protein (H-FABP) Elisa kit]
1. Preparation: Remove the reagent kit from the refrigerator and re-equilibrate at room temperature for 30 minutes.
2. Mixing solution: dilute the 20-fold concentrated washing solution with distilled water to the original one.
3. Add standard products and samples to be tested: take a sufficient number of enzyme-coated plates and fix them to the frame. Set up standard wells, test sample wells and blank control wells respectively. Add 50μL of standard product; first add 10μL of the sample to be tested, then add 40μL of sample diluent (that is, the sample is diluted 5 times); the blank control well is not added.
4. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes.
5. Wash the plate: discard the liquid, pat dry on the absorbent paper, fill each well with the washing liquid, let stand for 1min, shake off the washing liquid, pat dry on the absorbent paper, repeat the washing plate 4 times (you can also use the washing machine to press Instructions for washing the board).
6. Add enzyme-labeled working solution: add 50μL of enzyme-labeled working solution to each well, without adding blank control wells.
7. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes.
8. Plate washing: Discard the liquid, pat dry on the absorbent paper, fill each hole with the washing liquid, let stand for 1min, shake off the washing liquid, pat dry on the absorbent paper, repeat the plate washing 4 times (you can also use the washing machine to press Instructions for washing the board).
9. Color development: add 50μL of developer A solution to each well, and then add 50μL of developer B solution. Mix with a plate mixer for 30s (or shake gently by hand for 30s), and avoid color development at 37 ℃ for 15min .
10. Termination: Remove the enzyme labeling plate and add 50μL of stop solution to each well to stop the reaction (the color changes from blue to yellow)
11. Determination: Zero the blank holes, and within 15 minutes after termination, measure the absorbance (OD value) of each well with a wavelength of 450 nm.
12. Calculation: According to the concentration of the standard product and the corresponding OD value, calculate the linear regression equation of the standard curve, and then calculate the corresponding sample concentration on the regression equation according to the OD value of the sample. You can also use various application software to Calculation. The final concentration is the actual measured concentration times the dilution factor.
[Sample requirements of rat heart-shaped fatty acid binding protein (H-FABP) Elisa kit]
1. The sample cannot contain sodium azide (NaN3) because sodium azide (NaN3) is an inhibitor of horseradish peroxidase (HRP).
2. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided.
3. The sample should be fully centrifuged, without hemolysis and particles.
[Precautions for rat heart-shaped fatty acid binding protein (H-FABP) Elisa kit]
1. The experiment is carried out in strict accordance with the instructions, and the result of the experiment must be determined by the reading of the microplate reader.
2. If the enzyme-labeled coated board is not used up after opening, it should be put into a sealed bag and added with desiccant immediately.
3. It is recommended that all standards, samples and blank controls be tested in duplicate, and the average value is taken to reduce the experimental error.
4. Keep in mind that the sample has been diluted 5 times. The calculation result is multiplied by 5 to obtain the actual concentration of the sample.
5. The quantitative range of this kit is 37.5-1200pg / mL, beyond this range, it is calculated from the extension of the standard curve, not as an accurate quantitative result, please dilute it with a special diluent to determine the accurate result (within the range of 37.5-1200pg / mL) ), Multiplied by the total dilution factor is the final concentration of the sample.
6. If the color is too light, the substrate incubation time can be extended properly.
7. In order to avoid cross-contamination, the tips, samples and blank controls should be replaced with a new one for each addition; the common components such as enzyme working solution, sample diluent and substrate should be cantilevered, and they should not touch the microwells. ; Do not reuse the sealing film.
8. The kits are used within the warranty period, and different batches of reagents should not be mixed.
9. Substrate B is sensitive to light and avoid prolonged exposure to light.
[Rat Heart Fatty Acid Binding Protein (H-FABP) Elisa Kit Operating Procedure Summary]
Prepare reagents, samples and standards
Add prepared samples and standards, and react at 37 ℃ for 30 minutes
Wash the plate 4 times, add the enzyme reagent, and react at 37 ℃ for 30 minutes
Wash the plate 4 times, add color developing solutions A and B, and develop at 37 ℃ for 15 minutes
Add stop solution
Read OD value within 15 minutes
Calculation
[Rat heart fatty acid binding protein (H-FABP) Elisa kit detection range]
37.5pg / mL-1200pg / mL
[Specification of rat heart-shaped fatty acid binding protein (H-FABP) Elisa kit]
96 servings / box
ã€Storage of Rat Heart-shaped Fatty Acid Binding Protein (H-FABP) Elisa Kit】
Store at 2-8 ℃, protected from light and moisture.
ã€Effective period of rat heart-shaped fatty acid binding protein (H-FABP) Elisa kit】
6 months
High-altitude Operation Truck, Aerial work truck, Sky lift truck, Overhead working truck transport refers to the staff and equipment to the site to be used and air operations of special vehicles.
Through the big arm front bracket, can be quickly installed lifting device or platform, realize the material function such as lifting, lifting, and manned aerial work, at the same time to extension assignments device and a variety of work device quickly switch provides the interface.
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classification
According to the form of its lifting mechanism, generally can be divided into the telescopic arm type (ZhiBeiShi), folding arm type (crank arm type), vertical lift and hybrid four basic forms, etc.
Shear fork
Shear fork overhead working truck aerial work special equipment is widely used. The mechanical structure of it, make the lifting platform lifting has high stability, wide operating platform and high bearing capacity, larger, and the area of aerial work for many people work at the same time. It makes the work high above the more efficient, safe and more security.
Telescopic jib
Telescopic boom type aerial work platform is in the service in various industries aerial work, equipment installation, maintenance and other mobility aerial work products. Telescopic boom type aerial work platform is mainly divided into ZhiBeiShi aerial work platform and arm type aerial work platform. More used in the shipyard and other highly demanding place, this kind of machine safety is good, easy to move, but the cost is very high.
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The crank arm type aerial work platform
Set of cylinder type
Set of overhead working truck cylinder type is a multipurpose aerial work machinery, its function will be carrying workers and use of equipment platform to the specified height carries on the special engineering equipment of the air. This equipment is widely used in industrial workshop, tall hall, warehouse, station, the work high above the square, etc. The machine by the platform, telescopic oil cylinder, single or dual ladder ladder type type anti-rotating tank body, chassis, leg, wheel, etc. Have telescopic cylinder direct jack-up platform, when start the motor, driven by the motor gear pump oil, the oil valve and electromagnetic input channels with oil cylinder, telescopic cylinder is up one by one day, when the platform when the system pressure reaches the maximum height to reach the rated working pressure, at this time have the unloading overflow valve, oil pressure to keep work at constant pressure, after the stop check valve pressure maintaining, platform will stay in the largest working position, platform can be below the maximum height according to the height of the scene of the homework stay at any position.
Working device
Overhead working truck working device including the leg mechanism, the lifting mechanism, slewing mechanism, operation platform and leveling mechanism, etc.
(1) the leg mechanism
Currently use hydraulic leg more. Use of automobile engine out of power to drive the hydraulic pump, through the control valve hydraulic pump of hydraulic oil supply working cylinder of the hydraulic leg, leg is adjustable. On both sides of overhead working truck, general equipped with a joystick, can make the four hydraulic leg around alone extend or retract, so even on uneven or dumping ground, also can adjust the body to the level state, the safe operation.
The rise and fall of implementation work platform and transformer.
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(4) the work platform and leveling mechanism
The end connection of lifting mechanism of operation platform, is used for basic building blocks for the manned or equipment. In order to ensure the safety work and prevent equipment operation personnel, on the structure and performance of the work platform put forward specific requirements. Such as platform barrier height, width, non-slip surface platform, platform of seat belts, and so on.
In order to make the work platform at the bottom of the plane is always in the process of operation level, the work high above the car is equipped with automatic leveling work platform level institutions, mainly mechanical, liquid machine combination, electrohydraulic combined three categories.
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