Researchers at the University of Illinois have developed a method to create 3d images of living embryos in cattle, which may help determine embryo viability before in vitro fertilization.
A flash-rendered image of a bovine embryo cut through the center to reveal the internal structure. Image: Image courtesy Gabriel
Infertility is a devastating blow to those who want children. The cost of many people seeking treatment for a IVF cycle may be $20,000, which makes it desirable to succeed in as few attempts as possible. Advanced knowledge about embryo health can help doctors choose the people who are most likely to succeed in pregnancy.
The new method was published in the journal Nature Communications, and a collaboration project was completed by Professor Gabriel Posescu, a professor of electrical and computer engineering, and Matthew Wheeler, a professor of animal science, who was in the United States. The Beckman Institute of Advanced Science and Technology completed.
This method is called Gradient Light Interference Microscopy, which solves the difficulty of other methods for thick multi-cell samples. Under many conventional biomedical microscopes, light is produced through the tissue sheets of the sheet. Other methods use chemical or physical markers that allow the operator to find the specific object they are looking for in a thick sample, but these markers may be harmful to living tissue.
Mikhail Kandel, a co-author of the study, said: When you look at thick samples in other ways, your image will be washed away by the reflection of light from all surfaces in the sample. It's like watching a cloud. By controlling the path length of light passing through the specimen, GLIM can penetrate deep into thick samples. This technology allows researchers to take images from multiple depths and then synthesize a three-dimensional image.
To demonstrate this new approach, Popescu's team worked with Wheeler and his team to study cow embryos. One of the holy grails of embryology is to find a way to determine which embryos are most viable, Wheeler said: There is a non-invasive method associated with embryonic developmental capacity that is key, before Grim we tried more Experience and guess.
These well-founded guesses are made by examining factors such as the color of the fluid in the embryonic cells and the timing of development, but there is no universal marker to determine embryo health. This method allows us to see the entire image, just like the 3D model of the entire embryo.
However, choosing the healthiest embryo is not the end of the story. The final test result will prove that we have chosen a healthy embryo, and it has begun to develop a live calf.
Illinois has been conducting in vitro research with dairy cows since the 1950s. The resources provided by Gabriel's research and other resources of the Beckman Institute have become a perfect storm scenario.
The research team hopes to apply GLIM technology to human fertility research and treatment, as well as a range of different types of tissue research. Popescu plans to continue working with other biomedical researchers to successfully study thick samples of brain tissue from marine science in neuroscience research.
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